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  • 3M™ Crustacean Protein ELISA Kit, E96CRU, 96 wells/kit
  • 3M™ Crustacean Protein ELISA Kit, E96CRU, 96 wells/kit

    3M ID 70201175620
    • Designed to detect both processed and unprocessed target allergen proteins.
    • Test can be used for environmental swabs, CIP rinse water and food samples.
    • A universal extraction protocol simplifies testing for multiple analytes.
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    Specifications
    Brand
    3M™
    Manufacturing Segment
    Ready-to-eat
    Product Code
    E96CRU
    Product Type
    Test
    Storage Temperature
    36-46°F
    Test Type
    Allergen
    Details

    The 3M™ Crustacean Protein ELISA Kit is intended for the detection of crustacean proteins in clean-in-place (CIP) final rinse water, environmental swab samples, food ingredients and processed food products.

    The 3M Crustacean Protein ELISA Kit utilizes a sandwich ELISA. The crustacean proteins present in the sample react with the anti-crustacean antibody, which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-crustacean antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound crustacean protein. Following a second washing step, the enzyme bound to the immunosorbent is detected by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The color development from this enzymatic reaction varies directly with the concentration of crustacean protein in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of crustacean protein in the test sample. The quantity of crustacean protein in the test sample can be extrapolated from the standard curve, constructed from standards of known concentration, and adjusted to consider the sample dilution.

    • Designed to detect both processed and unprocessed target allergen proteins.
    • Test can be used for environmental swabs, CIP rinse water and food samples.
    • A universal extraction protocol simplifies testing for multiple analytes.